Antigen RhD in this disease, hemolytic infants plays an important role and IT field, where the determination of the RhD fetal with the use of invasive methods, such as by amniocentesis, etc. the dangers to mother and fetus will follow. in this study attempted to genotype RhD fetal DNA, fetal available in the serum of pregnant mothers, etc. for non-invasive, and the use of the method, Hemi-nested PCR to determine.